UJI AKTIVITAS ANTIOKSIDAN FRAKSI AIR EKSTRAK ETANOL DAUN KELOR (Moringa oleifera L.) DENGAN METODE DPPH (1,1-DIPHENYL-2-PICRYLHYDRAZYL) SERTA PENETAPAN KADAR FLAVONOID TOTALNYA

Firda, Indria (2018) UJI AKTIVITAS ANTIOKSIDAN FRAKSI AIR EKSTRAK ETANOL DAUN KELOR (Moringa oleifera L.) DENGAN METODE DPPH (1,1-DIPHENYL-2-PICRYLHYDRAZYL) SERTA PENETAPAN KADAR FLAVONOID TOTALNYA. Skripsi thesis, Universitas Wahid Hasyim Semarang.

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Abstract

Moringa leaf (Moringa oleifera L.) leaves are shurb that is mostly consumed by some people as vegetables are of the leaf kelor contenct is flavonoid that have potention as antioxidants. This study aims to determine the presence of antioxidant activity of water fraction ethanol extract of kelor leaves using method and to determine is total flavonoid content by spectrocolorimetric method. Simplicia powder was extracted by percolation method then fractionation was done using 96% ethanol as solvent. Antioxidant activity was measured using visible spectrophotometric at λ max 518,60 nm to obtain IC50 value with quercetin as a positive control. The determine of total flavonoid content used spetrocolorimetric method with AlCl3 as reagent. Moringa leaves had antioxidant activity based on the result IC50 fraction of water extract ethanol leaf was 168,44 μg / mL (IC50 quercetin 8,57μg / mL). Its antioxidant activity was weak compare to quercetin standart. The Total flavonoid content was 5,17 mgQE / g extract.

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