UJI AKTIVITAS ANTIBAKTERI MINYAK ATSIRI DAN EKSTRAK LIMBAH SISA DESTILASI RIMPANG KUNIR PUTIH (Kaempferia rotunda L.) TERHADAP PERTUMBUHAN Streptococcus pneumoniae

Rachma, Yauni Hidayatun (2018) UJI AKTIVITAS ANTIBAKTERI MINYAK ATSIRI DAN EKSTRAK LIMBAH SISA DESTILASI RIMPANG KUNIR PUTIH (Kaempferia rotunda L.) TERHADAP PERTUMBUHAN Streptococcus pneumoniae. Skripsi thesis, Universitas Wahid Hasyim Semarang.

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Abstract

White curcuma rhizome (Kaempferia rotunda L.) had antibacterial activity. In addition to essential oils, white curcuma rhizome also contains alkaloids, steroids, terpenoids, flavonoids and saponins, so there’s a possibilities that residual waste distillation had antibacterial activity. This study aims to observe antibacterial activity of the essential oil and residual waste distillation extracts of white curcuma rhizome against Streptococcus pneumoniae. Essential oil was obtained by water and steam distillation, while residual waste distillation was extracted by soxhlet using 96% ethanol. The antibacterial activity was tested by agar diffusion method. The series of essential oil concentration 7.5; 8.0; 8.5 and 9.0% (v/v) and residual waste distillation extract at concentration 2.0; 2.5; 3.0 and 4.0 mg/disk. Erythromycin 15 µg/disc as positive control while negative control used PEG 400 and DMSO. The zone of inhibition value was statistically analysed using Kruskal Wallis and T-test independent. The result showed that essential oil and residual waste distillation extracts of white curcuma rhizome had antibacterial activity against Streptococcus pneumoniae. Differences of zone of inhibition value between serial concentration essential oil was seen at 7.5% and 9.0% v/v, but serial concentrations in residual waste distillation extracts didn’t significantly difference. Antibacterial activity of essential oils and residual waste distillation extracts was significantly different (sig.<0.05) against Streptococcus pneumoniae.

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