EFEK SITOTOKSIK EKSTRAK METANOL DAUN TALAS (Colocasia esculenta L. Schott) TERHADAP SEL MCF-7 MELALUI INDUKSI APOPTOIS

Khadijah, Gina Puspita (2018) EFEK SITOTOKSIK EKSTRAK METANOL DAUN TALAS (Colocasia esculenta L. Schott) TERHADAP SEL MCF-7 MELALUI INDUKSI APOPTOIS. Skripsi thesis, Universitas Wahid Hasyim Semarang.

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Abstract

Breast cancer is one of the major causes deaths of women around the world. Taro is a plant that has anticancer activity. Taro leaf contain flavonoids that can induce cancer cell apoptosis. MCF-7 cell is one model of breast cancer cells that widely used in research. The aim of this study was to prove the cytotoxic effect of taro leaf methanol extract on MCF-7 cells through apoptosis induction. Taro leaf was extracted with soxhletation method using methanol as a solvent. Cytotoxicity test was done according to MTT assay with extract concentration series which were 1000; 500; 250; 125; 62,5 µg/ ml against MCF-7 cells. Viability percentage data of the MCF-7 cells was used to establish IC 50 value by using probit analysis through SPSS 16 for Windows. The apoptosis test used a double-staining method with an etidium bromide-acridine orange reagent. The result showed that methanol extract of taro leaf had cytotoxic effect to the MCF-7 cells. IC 50 value was 331,838 μg/ml for MCF-7 cells. Apoptosis test results showed that methanol extract of taro leaf is able to induce apoptosis of MCF-7 cells.

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