EFEK EKSTRAK ETANOLIK RIMPANG TEMU KUNCI (Boesenbergia pandurata) TERHADAP AKTIVITAS SITOTOKSIK 5-FLOUROURASIL PADA SEL KANKER KOLON WiDr

Sulistiyawati, Sulistiyawati (2018) EFEK EKSTRAK ETANOLIK RIMPANG TEMU KUNCI (Boesenbergia pandurata) TERHADAP AKTIVITAS SITOTOKSIK 5-FLOUROURASIL PADA SEL KANKER KOLON WiDr. Skripsi thesis, Universitas Wahid Hasyim Semarang.

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Abstract

5-Flourourasil is a commonly used chemotherapeutic agent in the colon cancer treatment but has adverse side effects and the risk of resistance. One of many efforts to handle it is combined with chemopreventive agent. Cytotoxic activities on colon cancer cells WiDr have been found in the ethanolic extract of temu kunci rhizome. Panduratin A is one of the compounds contained in the temu kunci rhizhome that has been shown to have cytotoxic activity against some cancer cells. The purpose of this research is to know the synergistic effect of ethanolic extract of rimpang temu kunci and 5-flourourasil on colon cancer WiDr. This type of research was laboratory experimental. The Temu kunci rhizome was extracted by maceration method using 70% ethanol solvent. The cytotoxic test of ethanolic extract of single and combination concoction with 5-Flourourasil against collon cancer cell WiDr was done by MTT assay methode. Data obtained from cytotoxic test and combination of live cell percentage. Data analysis was performed using probit analysis on SPSS 16 for Windows between live cell percentage and log concentration to obtain IC50 value. The combined index (CI) was calculated to determine whether the resulting effect was synergistic, additive or antagonistic. The results showed that ethanolic extract of temu kunci rhizome had cytotoxic effect shown by IC50 value of 48,871 μg/ml and 5-Flourourasil 828,833 μM. The combination of ethanolic extract of temu kunci rhizome with 5-Flourourasil produced antagonistic effect.

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